Journal: Frontiers in Microbiology
Article Title: Lactobacillus paracasei WIS43 alleviates DSS-induced colitis by modulating gut microbiota and suppressing inflammation
doi: 10.3389/fmicb.2025.1721585
Figure Lengend Snippet: Effects of Lactobacillus paracasei interventions on gut microbiota composition in DSS-induced colitis mice. (A) Alpha diversity indices (Chao1, Shannon, Simpson). WIS43 significantly increased Shannon and Simpson indices compared with DSS, indicating partial restoration of microbial richness and evenness. (B) Principal coordinate analysis (PCoA) based on Bray–Curtis distances revealed distinct clustering of microbial communities among groups (PERMANOVA, R 2 = 0.33, p = 0.001). (C) Non-metric multidimensional scaling (NMDS) analysis confirmed separation of microbial compositions, with a stress value of 0.176, indicating reliable ordination. For microbiota analysis, n = 6 samples were randomly selected from each group. (D,E) Relative abundance of gut microbiota at the genus (D) and species (E) levels. WIS43 restored the abundance of beneficial taxa such as L. johnsonii and L. animalis while reducing potentially pathogenic taxa including Kineothrix and Duncaniella .
Article Snippet: The neighbor-joining tree demonstrated that WIS43 clustered robustly with L. paracasei lineages, forming a well-supported clade with L. paracasei subsp. paracasei ATCC 25302 and L. paracasei subsp. tolerans JCM 1171, with bootstrap values exceeding 90% ( ).
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